Composite
Part:BBa_K539741:Design
Designed by: Yang, Yi-Pei Group: iGEM11_NCTU_Formosa (2011-09-22)
TetR repressible promoter (BBa_R0040)+ kivd (alpha-ketoisovalerate decarboxylase)+Ter (BBa_J61048)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 658
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1735
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Kivd is under tetR repressible promoter that is continuously turn ON or repressed by tetR.
Source
Kivd is cloned form Lactococcus lactis subsp, lactis KF147, by Zeng lab, NCTU-Formosa.